The painting shows the Biblical Christ, Jesus the Saviour crucified, and seemingly lifeless. The cross stands on a rock, and a skull is underneath. The interpretation is that the place is Golgotha, "the Place of the Skull." The area signifies death, and it's no wonder the Saviour is about to die here. On the left are roman soldiers holding Jesus' cloth. They seem to argue about who is going to take it. The scenario is really sad. Peter, one of the closest disciples of Jesus and a saint, watches in deep concern and sadness. He is grieved by the death of His friend, as displayed by the cries of angels hovering above Jesus.

Towards the left is a scene that juxtaposes with that on the right. While the Roman soldiers seem to have made an achievement, the pious and devout on the left are mourning. Mary mother of Jesus is in deep grief and can barely stand without the support of holy women and St. John. Mary Magdalene bends over Jesus' feet, whose wounds are bloody from the nailing. The Crucifixion by Giotto was inspired by the piety life and the devastating departure of Jesus. The Franciscans also played a significant role in influencing Giotto, since they were striving hard to live the lowly life of Jesus, and to be perfect examples in practising his teachings.

A person looking at the picture gets the feel of firsthand experience as a witness of the advents and feelings that engulf the atmosphere. Christ's face is upon the Holy ones who are on the left of the picture. This gesture shows Jesus's compassion towards His faithful followers, which lights some hope in the sense that it's not over, and Christ is going to save them somehow, even in this situation. Jesus has a halo that shines on his head and not a crown of thorns. The halo signifies glory, which also appears on all the other saints. The halo also juxtaposes with the situation of the devout, who are in deep sorrow.

The armed and celebratory soldiers are disregarded since they are not expected to argue about who would take Jesus's cloth. It is shameful and unworthy of them. One of the soldiers is shoe-less, which contradicts with the power displayed by the swords in their hands. The Crucifixion by Giotto carries lots of contrast that is widely used to differentiate two groups. The cross at the centre cuts across the worlds of the people with precision. The symmetry is perfect, not even the number of angels (five) on each side of the cross deviates.